微管关联蛋白1轻链3β(MAP1LC3b)重组蛋白 

Recombinant Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b)

MAP1A/1BLC3; ATG8F; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: 1-125

Tags: N-terminal His Tag

Subcellular Location: Membrane, Cytoplasm

Purity: ＞95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl,

5%Trehalose.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 17.7kDa

Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not

vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8ºC for one month.

 Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined

by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no

obvious degradation and precipitation were observed. The loss rate is less than 5% within the

expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure. Gene Sequencing (Extract)

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