Human HGF Protein, His Tag (MALS verified)
分子别名(Synonym)
HGF,HPTA,SF
表达区间及表达系统(Source)
Human HGF, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 .
Predicted N-terminus: Gln 32
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90kDa (alpha & Beta chain), 58-63 (alpha chain), 33 kDa (Beta chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Human HGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human HGF, His Tag is more than 90% and the molecular weight of this protein is around 95-105 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human HGF, His Tagt 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag with a linear range of 1-39 ng/mL (QC tested).
Protocol
活性(Bioactivity)-SPR
Human HGF R Protein, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF Protein, His Tag with an affinity constant of 75.2 pM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
活性(Bioactivity)-BLI
Loaded Human HGF R, Fc Tag on Protein A Biosensor, can bind Human HGF, His Tag with an affinity constant of 3.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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