Mouse BAFF / TNFSF13B / CD257 Protein, Tag Free
分子别名(Synonym)
TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1
表达区间及表达系统(Source)
Mouse BAFF, Tag Free s expressed from human 293 cells (HEK293). It contains AA Ala 127 - Leu 309
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries no "tag".
The protein has a calculated MW of 20.9 kDa. The protein migrates as 20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Mouse BAFF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
活性(Bioactivity)-ELISA
Immobilized Mouse BAFF, Tag Freeat 2 μg/mL (100 μL/well) can bind Mouse BAFFR, Fc Tag with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
Immobilized Mouse BAFF, Tag Free at 5 μg/mL (100 μL/well) can bind Mouse BCMA, Fc Tag with a linear range of 0.8-13 ng/mL (Routinely tested).
Protocol
Immobilized Mouse BAFF, Tag Free at 5 μg/mL (100 μL/well) can bind Human TACI/TNFRSF13B, Fc Tag with a linear range of 0.8-13 ng/mL (Routinely tested).
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