Mouse TRAIL R2 / DR5 / TNFRSF10B Protein, His Tag
分子别名(Synonym)
TNFRSF10B,TRAILR2,TRAIL-R2,CD262,DR5,KILLER,TRICK2,ZTNFR9,TRICKB
表达区间及表达系统(Source)
Cynomolgus TRAIL R2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 58 - Ser 212.
Predicted N-terminus: Ile 58
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.1 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Cynomolgus TRAIL R2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Cynomolgus TRAIL R2, His Tag is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Cynomolgus TRAIL R2, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 2-63 ng/mL (QC tested).
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