Human CD30 Ligand / TNFSF8 Protein, His Tag, low endotoxin

分子别名（Synonym）

CD30-L,CD153,TNFSF8,CD30L,CD30LG,CD153 antigen,CD30 antigen ligand,CD30 Ligand

表达区间及表达系统（Source）

Human CD30 Ligand, His Tag, low endotoxin  is expressed from human 293 cells (HEK293). It contains AA Gln 63 - Asp 234.

Predicted N-terminus: His

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 21.5 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human CD30 Ligand, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性（Bioactivity）-ELISA

Immobilized Human CD30, Fc Tag at 2 μg/mL (100 μL/well) can bind Human CD30 Ligand, His Tag, low endotoxin  with a linear range of 0.039-0.313 μg/mL (QC tested).

Protocol

Immobilized Human CD30 Ligand, His Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human CD30, Llama IgG2b Fc Tag, low endotoxin with a linear range of 0.6-10 ng/mL (Routinely tested).

Protocol

活性（Bioactivity）-BLI

Loaded Biotinylated Human CD30, Avitag,His Tag  on SA Biosensor, can bind Human CD30 Ligand, His Tag, low endotoxin with an affinity constant of 132 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

活性（Bioactivity）-FACS

FACS analysis shows that Human CD30 Ligand, His Tag, low endotoxin can bind to K562-CD30 cells surface CD30. The concentration of Human CD30 Ligand is 3 μg/mL (Routinely tested).

Protocol

FACS analysis shows that the binding of Human CD30 Ligand, His Tag, low endotoxin  to K562-CD30 cells surface CD30 was inhibited by increasing concentration of neutralizing anti-CD30 antibody. The concentration of Human CD30 Ligand used is 3 μg/mL. The IC50 is 0.2401 μg/mL (Routinely tested).

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