Human HVEM / TNFRSF14 Protein, Strep Tag

分子别名（Synonym）

TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270

表达区间及表达系统（Source）

Human HVEM, Strep Tag  is expressed from human 293 cells (HEK293). It contains AA Leu 39 - Val 202

Predicted N-terminus: Leu 39

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a twin strep tag at the C-terminus.

The protein has a calculated MW of 20.4 kDa. The protein migrates as 27-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human HVEM, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性（Bioactivity）-ELISA

Immobilized Human HVEM, Strep Tagat 5 μg/mL (100 μL/well) can bind Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.3-5 ng/mL (QC tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！