Human IFN-alpha / beta R1 Protein, Fc Tag

分子别名（Synonym）

IFNAR1,IFNAR,CRF2-1,IFN-R-1,IFNAR-1

表达区间及表达系统（Source）

Human IFNAR1, Fc Tag  is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436.

Predicted N-terminus: Lys 28

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 73.8 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IFNAR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性（Bioactivity）-BLI

Loaded Human IFN-alpha 1, His Tagon HIS1K Biosensor, can bind Human IFNAR1, Fc Tag  with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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