Human CD117 / c-kit Protein, Fc Tag (MALS verified)

分子别名（Synonym）

CD117,SCFR,c-Kit,KIT

表达区间及表达系统（Source）

Human CD117, Fc Tag is expressed from human 293 cells. It contains AA Gln 26 - Thr 516 ).

Predicted N-terminus: Gln 26

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 81.9 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human CD117, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

The purity of Human CD117, Fc Tag is more than 85% and the molecular weight of this protein is around 185-225 kDa verified by SEC-MALS.

Report

活性（Bioactivity）-ELISA

Immobilized ActiveMax® Human SCF, Tag Free  at 2 μg/mL (100 μL/well) can bind Human CD117, Fc Tag with a linear range of 0.4-2 ng/mL (QC tested).

Protocol

Immobilized Human CD117, Fc Tag  at 2 μg/mL (100 μL/well) can bind Mouse SCF, His Tag  with a linear range of 0.05-1 ng/mL (Routinely tested).

Protocol

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！