Human Latent Activin A / INHBA Protein, His Tag

分子别名（Synonym）

Activin A,INHBA

表达区间及表达系统（Source）

Human Latent Activin A, His Tags expressed from human 293 cells . It contains AA Ser 21 - Ser 426 .

Predicted N-terminus: His (pro) & Gly311 (mature)

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 13.0 kDa (mature) and 32.0 kDa (pro). As a result of glycosylation and Interchain disulfide bond, the protein migrates as 15 kDa (mature) and 43-48 kDa (pro) under reducing (R) condition, and 27 kDa (mature), 43-48 kDa (pro) and 60 kDa (pro & mature) under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human Latent Activin A, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Immobilized Human Latent Activin A, His Tag  at 10 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag  with a linear range of 0.6-20 ng/mL (QC tested).

Protocol

活性（Bioactivity）-Cell based assay

The bio-activity of Human Latent Activin A, His Tag was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically 47-66 ng/mL (Routinely tested).

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