ActiveMax® Human Activin A / INHBA Protein, Tag Free, low endotoxin

分子别名（Synonym）

Activin A,INHBA

表达区间及表达系统（Source）

ActiveMax® Human Activin A, Tag Free, low endotoxin is expressed from human 293 cells . It contains AA Gly 311 - Ser 426 .

Predicted N-terminus: Gly 311

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 13.0 kDa. As a result of glycosylation, the protein migrates as 14-15 kDa under reducing (R) condition, and 23-25 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 30% ACN, 0.056% TFA. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

ActiveMax® Human Activin A, Tag Free, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human Activin A, Tag Free, low endotoxin at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag  with a linear range of 0.4-25 ng/mL (QC tested).

Protocol

活性（Bioactivity）-Cell based assay

The bio-activity of ActiveMax® Human Activin A, Tag Free, low endotoxin  was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically ≤ 9.5 ng/mL

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