Human Activin RIIA / ACVR2A Protein, Fc Tag
分子别名(Synonym)
Activin receptor type IIA,ACTRIIA
表达区间及表达系统(Source)
Human ACVR2A, Fc Tag is expressed from human 293 cells. It contains AA Ala 20 - Pro 135 .
Predicted N-terminus: Pro
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 39.9 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Human ACVR2A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
活性(Bioactivity)-ELISA
Immobilized ActiveMax® Human Activin A, Tag Free, low endotoxin at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag with a linear range of 0.4-25 ng/mL (QC tested).
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